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This laboratory project uses the context of Acid Mine Drainage to teach concepts important to analytical chemistry and quantitative analysis. Students set up experiments that mimic the process of metal sulfide mineral oxidative dissolution. The experiments explore how the rate of dissolution changes with respect to changes in pH, added oxidizing agents, and oxygen rich or oxygen poor environments. Visible spectroscopy is used to initially measure the concentration of complexed iron in solution. ICP-AES is used to verify the stoichiometry of the arsenopyrite sample. Elemental sulfur determination and the speciation of the aqueous sulfur in the solution can be determined using reverse phase and ion pair high performance liquid chromatography.


Table of Contents

  • Instructors Manual (Word, PDF)
  • Project Overview (Word, PDF)
  • PowerPoint Presentation for Lecture on AMD Problem (PowerPoint, PDF)
  • Project Introduction Assignment (Word, PDF)
  • Progress Report (Word, PDF)
  • Final Paper and Oral Report Instructions (Word, PDF)
  • Final Paper Grading Rubric (Word, PDF)
  • Individual Grade Rubric (Word, PDF)
  • Peer Evaluation Document (Word, PDF)
  • Appendices
    • Spectrophotometric Determination of Fe-Student Lab (Word, PDF)
    • Spectrophotometric Determination of Fe-Instructor notes and prep notes (Word, PDF)

More detailed supplemental materials and solutions to some of the problems presented are available. Contact Pamela Doolittle ( for more information.

Developed by:

Dr. Pamela Doolittle
(Main Contact)
University of Wisconsin-Madison

Dr. Robert J. Hamers
University of Wisconsin-Madison

“This project evolved from a research project supervised by Robert Hamers. That work was funded by grants from the National Science Foundation (CHE 9807598) and the Environmental Protection Agency (R 826189).”

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