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This module provides an introduction to Affinity Chromatography separations. This is a technique commonly used to purify a biomolecule of interest based on the specific interaction of the biomolecule with a ligand immobilized on a stationary phase.Affinity chromatography is widely used in biotechnology research and in industry. Using this technique, molecules of interest can be purified in a one-step separation with high purity and high recovery. The purification of the molecule does not require harsh conditions and hence is preferred for biomolecule separation.

Affinity chromatography has also found applications in studying drug/ligand binding to proteins of interest. This technique is useful I the purification of peptides, chiral molecules, antibodies, nucleic acids, and proteins.

The aim of this module is to present the basic theory and applications of affinity chromatography. This module is meant to be utilized in the classroom as a teaching resource or by anyone interested in learning about affinity chromatography. As a teaching resource, the module can be used directly, or the students can be given an in-class problem set, which will lead to further discussion on affinity chromatography.


Developed by:
Dr. Sapna Deo
University of Miami

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