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After completing this activity, students will be able to:

  • Describe the relationship between absorbance and transmittance.
  • Develop a proportional relationship between absorbance and concentration.
  • Account for the role of sample pathlength in absorbance measurements.
  • Determine the molar absorptivity of a compound based on its absorbance behavior according to Beer’s Law.
  • Construct a spectrum from absorbance measurements at various concentrations.

Kate Mullaugh, College of Charleston (

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