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High Performance Liquid Chromatography

HomeAll Posts14. High Performance Liquid Chromatography
Separation Science

Separation Science

00. Complete Active Learning Units with Supporting Text, a. Basic chromatographic concepts, a. General Coverage of GC, a. General Coverage of HPLC, a. Ion Chromatography, b. GC Retention Order, b. HPLC Retention Order, b. Liquid-liquid extraction, b. Steric Exclusion chromatography, c. Peak broadening, d. Capillary electrophoresis, d. Fundamental resolution equation, In Class Activities, Textual Material
A series of collaborative learning activities and accompanying text that develop the field of separation science, with a particular emphasis on chromatographic separations, is provided. These activities are intended to be done in class by students working in groups, but can be modified for use as out-of-class exercises. Learning objectives, an instructor’s manual, and out-of-class problems…

Chromatography: Use of an HPLC Simulator

19. Simulations, a. General Coverage of HPLC, b. HPLC Retention Order, c. Use of a Simulator, In Class Activities
A series of collaborative learning activities using the free HPLC Simulator (http://www.multidlc.org/hplcsim) developed by Thomas J. Lauer and Dwight R. Stoll of Gustavus Adolphus University. The Chromatography Basics activities introduce the parameters tR, tM, k, resolution, peak width, N, H, Purnell equation for resolution, van Deemter curve, linear velocity, optimum linear velocity, and optional discussion…

Developing an Analytical Method for the Analysis of a Medicinal Plant

18. Analytical Method Development, 22. Contextual Modules, b. Sample preparations, Contextual Modules, d. HPLC Method Development
Introduction This module introduces students to the process of developing an analytical method using, as a case study, the quantitative analysis of eight analytes in the medicinal plant Danshen using a combination of a microwave extraction to isolate the analytes and HPLC with UV detection to determine their concentrations. The module is divided into nine parts:…
Interpreting primary literature

Interpreting the Primary Literature

20. Activities that Use Primary Literature, a. Basic chromatographic concepts, b. Acid-base equilibria, d. GC-MS, e. HPLC - Literature Article Analysis, e. Mass Spec - Literature Article Analysis, e. Voltammetric Methods, f. GC - Literature Article Analysis, f. Method selection in chromatography, h. Atomic Spectroscopy, In Class Activities
Introduction These assignments are designed to be capstone activities at the end of units on figures of merit (such as sensitivity and LOD), acid-base equilibria, separations, spectroscopy, mass spectrometry, and electrochemistry. Each assignment consists of an out-of-class reading assignment drawn from the primary literature accompanied by objective questions and a set of open-ended, in-class discussion questions.…