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General Coverage of HPLC

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Separation Science

Separation Science

00. Complete Active Learning Units with Supporting Text, a. Basic chromatographic concepts, a. General Coverage of GC, a. General Coverage of HPLC, a. Ion Chromatography, b. GC Retention Order, b. HPLC Retention Order, b. Liquid-liquid extraction, b. Steric Exclusion chromatography, c. Peak broadening, d. Capillary electrophoresis, d. Fundamental resolution equation, In Class Activities, Textual Material
A series of collaborative learning activities and accompanying text that develop the field of separation science, with a particular emphasis on chromatographic separations, is provided. These activities are intended to be done in class by students working in groups, but can be modified for use as out-of-class exercises. Learning objectives, an instructor’s manual, and out-of-class problems…

Chromatography: Use of an HPLC Simulator

19. Simulations, a. General Coverage of HPLC, b. HPLC Retention Order, c. Use of a Simulator, In Class Activities
A series of collaborative learning activities using the free HPLC Simulator (http://www.multidlc.org/hplcsim) developed by Thomas J. Lauer and Dwight R. Stoll of Gustavus Adolphus University. The Chromatography Basics activities introduce the parameters tR, tM, k, resolution, peak width, N, H, Purnell equation for resolution, van Deemter curve, linear velocity, optimum linear velocity, and optional discussion…