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HPLC Retention Order

Separation Science

Separation Science

A series of collaborative learning activities and accompanying text that develop the field of separation science, with a particular emphasis on chromatographic separations, is provided. These activities are intended to be done in class by students working in groups, but can be modified for use as out-of-class exercises. Learning objectives, an instructor’s manual, and out-of-class problems…

Chromatography: Use of an HPLC Simulator

A series of collaborative learning activities using the free HPLC Simulator ( developed by Thomas J. Lauer and Dwight R. Stoll of Gustavus Adolphus University. The Chromatography Basics activities introduce the parameters tR, tM, k, resolution, peak width, N, H, Purnell equation for resolution, van Deemter curve, linear velocity, optimum linear velocity, and optional discussion…