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Specialty Separation Methods

HomeAll Posts15. Specialty Separation Methods
Separation Science

Separation Science

00. Complete Active Learning Units with Supporting Text, a. Basic chromatographic concepts, a. General Coverage of GC, a. General Coverage of HPLC, a. Ion Chromatography, b. GC Retention Order, b. HPLC Retention Order, b. Liquid-liquid extraction, b. Steric Exclusion chromatography, c. Peak broadening, d. Capillary electrophoresis, d. Fundamental resolution equation, In Class Activities, Textual Material
A series of collaborative learning activities and accompanying text that develop the field of separation science, with a particular emphasis on chromatographic separations, is provided. These activities are intended to be done in class by students working in groups, but can be modified for use as out-of-class exercises. Learning objectives, an instructor’s manual, and out-of-class problems…

Affinity Chromatography

c. Affinity chromatography, Textual Material
This module provides an introduction to Affinity Chromatography separations. This is a technique commonly used to purify a biomolecule of interest based on the specific interaction of the biomolecule with a ligand immobilized on a stationary phase.Affinity chromatography is widely used in biotechnology research and in industry. Using this technique, molecules of interest can be purified…

Effects of Acid Rain on Atlantic Salmon Populations

22. Contextual Modules, a. Acid-base titrations, a. Designing a sampling plan, a. Ion Chromatography, b. Complexation titrations, b. Sample preparations, Contextual Modules
Introduction This module provides a real world context for introducing fundamental quantitative techniques (pH, Ion Selective Electrodes, Ion chromatography, and Titrimery) used in chemical analysis of water samples. Using active learning pedagogy, module explores sampling and analyzing the base inorganic ions and acidity parameters in freshwater samples. The module frames the study using the…
Interpreting primary literature

Interpreting the Primary Literature

20. Activities that Use Primary Literature, a. Basic chromatographic concepts, b. Acid-base equilibria, d. GC-MS, e. HPLC - Literature Article Analysis, e. Mass Spec - Literature Article Analysis, e. Voltammetric Methods, f. GC - Literature Article Analysis, f. Method selection in chromatography, h. Atomic Spectroscopy, In Class Activities
Introduction These assignments are designed to be capstone activities at the end of units on figures of merit (such as sensitivity and LOD), acid-base equilibria, separations, spectroscopy, mass spectrometry, and electrochemistry. Each assignment consists of an out-of-class reading assignment drawn from the primary literature accompanied by objective questions and a set of open-ended, in-class discussion questions.…

Capillary Electrophoresis

d. Capillary electrophoresis
Students explore aspects of capillary electrophoresis that include methods of migration and variables that influence retention order. There is an associated text that explains the answers to the questions on the worksheet.

Ion Exchange Chromatography

a. Ion Chromatography
Students explore aspects of ion exchange chromatography including variables that affect retention order and methods of detection. There is an associated text that explains the answers to the questions on the worksheet.
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