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Specialty Separation Methods

Separation Science

Separation Science

A series of collaborative learning activities and accompanying text that develop the field of separation science, with a particular emphasis on chromatographic separations, is provided. These activities are intended to be done in class by students working in groups, but can be modified for use as out-of-class exercises. Learning objectives, an instructor’s manual, and out-of-class problems…

Affinity Chromatography

This module provides an introduction to Affinity Chromatography separations. This is a technique commonly used to purify a biomolecule of interest based on the specific interaction of the biomolecule with a ligand immobilized on a stationary phase.Affinity chromatography is widely used in biotechnology research and in industry. Using this technique, molecules of interest can be purified…

Effects of Acid Rain on Atlantic Salmon Populations

Introduction This module provides a real world context for introducing fundamental quantitative techniques (pH, Ion Selective Electrodes, Ion chromatography, and Titrimery) used in chemical analysis of water samples. Using active learning pedagogy, module explores sampling and analyzing the base inorganic ions and acidity parameters in freshwater samples. The module frames the study using the…
Interpreting primary literature

Interpreting the Primary Literature

Introduction These assignments are designed to be capstone activities at the end of units on figures of merit (such as sensitivity and LOD), acid-base equilibria, separations, spectroscopy, mass spectrometry, and electrochemistry. Each assignment consists of an out-of-class reading assignment drawn from the primary literature accompanied by objective questions and a set of open-ended, in-class discussion questions.…

Capillary Electrophoresis

Students explore aspects of capillary electrophoresis that include methods of migration and variables that influence retention order. There is an associated text that explains the answers to the questions on the worksheet.

Ion Exchange Chromatography

Students explore aspects of ion exchange chromatography including variables that affect retention order and methods of detection. There is an associated text that explains the answers to the questions on the worksheet.