Introduction
A series of collaborative learning activities and accompanying text that develop the concept of concentration calibration, utilizing the external standard, internal standard, and standard addition methods. The module is based primarily on flavonoids, particularly quercetin, as an example analyte. The activities are designed as in-class, small group exercises. An additional out-of-class activity is also available.…
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A variety of common standardization methods are developed in this activity.
Students explore calculations of the concentration on an unknown under a variety of different conditions and standardization methods.
Students explore a variety of calibration procedures when completing this activity.
Students learn to:
Identify different methods to calibrate instrumentation or analytical methodologies
Choose proper calibration method depending on sample characteristics or instrument.
Describe how standards and/or samples are prepared for each calibration method.
Calculate concentration from information obtained from each calibration method
Students explore a variety of calibration methods when completing this activity.
Students learn to:
explain the differences between external standard and standard addition calibration.
identify when the use of an internal standard is necessary.
discuss the merits and drawbacks of each type of calibration.
calculate the concentration of an analyte given…
Students explore a variety of calibration methods when completing this activity.
Students learn to:
Explain the purpose of using internal standards
Use response factors to calculate an unknown concentration based on an internal standard
Determine the limits of detection (LOD) and quantification of a method.