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After completing this activity, students will be able to:

  • Differentiate different types of columns (i.e., WCOT, PLOT, packed), their dimensions, applicability and diffusion factors affecting the separation
  • Evaluate suitability of common stationary phases for different analytes
  • Distinguish types of interactions between the analytes and stationary phase
  • Justify the selection of a stationary phase based on its interactions, stability, and bleed with the targeted analyte
  • Evaluate and explain the column bleed

Alena Kubatova, University of North Dakota (alena.kubatova@und.edu)