After completing this activity, students will be able to:
- Differentiate different types of columns (i.e., WCOT, PLOT, packed), their dimensions, applicability and diffusion factors affecting the separation
- Evaluate suitability of common stationary phases for different analytes
- Distinguish types of interactions between the analytes and stationary phase
- Justify the selection of a stationary phase based on its interactions, stability, and bleed with the targeted analyte
- Evaluate and explain the column bleed
Alena Kubatova, University of North Dakota (alena.kubatova@und.edu)