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After completing this exercise, students will be able to:

  1. Predict and explain the elution order of analytes.
  2. Explain the effects of temperature programs and flow rate on resolution at the macroscopic and molecular level.
  3. Explain the effects of flow rate.
  4. Select separation conditions for a given set of molecules. 

Rosalynn Quinones-Fernandez, Marshall University (quinonesr@marshall.edu)