After completion of this activity, students will be able to:
- Predict elution order of compounds
- Calculate retention factor and selectivity factor
- Explain the effect of mobile phase composition on resolution and peak shape at a molecular level
- Use elution gradients to improve separation of compounds in a mixture
- Explain the effect of flow rate on separation at a molecular level
Leonard Demoranville (Leonard.demoranville@centre.edu) and Daniel Scott (daniel.scott@centre.edu), Centre College
