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After completion of this activity, students will be able to:

  • Predict elution order of compounds
  • Calculate retention factor and selectivity factor
  • Explain the effect of mobile phase composition on resolution and peak shape at a molecular level
  • Use elution gradients to improve separation of compounds in a mixture
  • Explain the effect of flow rate on separation at a molecular level

Leonard Demoranville ( and Daniel Scott (, Centre College

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