Riboflavin HPLC Data

The data set available here is from the HPLC analysis of of riboflavin in urine. The Excel spreadsheet contains chromatographic data using a fluorescence detector with excitation at a wavelength of 340 nm and detection at a wavelength of 450 nm, and chromatographic data using a UV-Vis detector at a wavelength of 450 nm.

File: RiboflavinHPLC
Tab 1: Fluorescence (time, % fluorescence)
Tab 2: UVVis (time, absorbance)

The following figure provides one presentation of the data that demonstrates the difference between the selectivity of the two detectors. The peak marked with the red asterisk is riboflavin.

Data provided by Jason Schultz, Jonna Berry, Kaelene Lundstrom, and Dwight Stoll, Department of Chemistry, Gustavus Adolphus College.

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