Determining Absorbance in IR Spectroscopy

A quantitative analysis based on the absorption of infrared radiation, although important, is less frequently encountered than those for UV/Vis absorption. One reason is the difficulty in establishing a 100% T (A = 0) baseline as the optical properties of NaCl sample cells may change significantly with wavelength due to contamination and degradation. We can minimize this problem by measuring absorbance relative to a baseline established for the absorption band, as shown here.


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