HPLC Column

The most common type of HPLC column is a stainless steel tube with an internal diameter between 2.1 mm and 4.6 mm and a length between 30 mm and 300 mm. The column is packed with 3–10 μm porous silica particles with either an irregular or a spherical shape. Typical column efficiencies are 40,000–60,000 theoretical plates/m. Assuming a Vmax/Vmin of approximately 50, a 25-cm column with 50,000 plates/m has 12,500 theoretical plates and a peak capacity of 110.

The column shown here has an internal diameter of 4.6 mm and a length of 150 mm, and is packed with 5 μm particles coated with stationary phase.

Figure12.39

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