Instrumentation for Turbidimetry and Nephelometry

Turbidimetry and nephelometry are two techniques based on the elastic scattering of radiation by a suspension of colloidal particles: (a) in turbidimetry the detector is placed in line with the source and the decrease in the radiation’s transmitted power is measured.; (b) in nephelometry scattered radiation is measured at an angle of 90o to the source. The similarity of turbidimetry to the measurement of absorbance and of nephelometry to the measurement of fluorescence is evident in these instrumental designs . In fact, turbidity can be measured using a UV/Vis spectrophotometer and a spectrofluorimeter is suitable for nephelometry.

Figure10.64

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