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Tag Archives: absorbance
Methods for Improving Precision in Absorbance Measurements
As shown here, the relative uncertainty in determining absorbance (and thus in determining concentration) is greater at very high and very low absorbances (that is, when very little or almost all of the source radiation reaches the detector. One way … Continue reading
Posted in Illustration
Tagged absorbance, indeterminate error, precision, spectroscopy, uncertainty
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Determining Absorbance in IR Spectroscopy
A quantitative analysis based on the absorption of infrared radiation, although important, is less frequently encountered than those for UV/Vis absorption. One reason is the difficulty in establishing a 100% T (A = 0) baseline as the optical properties of … Continue reading
Beer’s Law
Absorbance is the more common unit for expressing the attenuation of radiation because it is a linear function of the analyte’s concentration. When monochromatic electromagnetic radiation passes through an infinitesimally thin layer of sample of thickness dx, it experiences a decrease … Continue reading
Posted in Illustration
Tagged absorbance, Beer's law, molar absorptivity, spectroscopy
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Absorbance and Emission Spectroscopy
The illustration here is a simplified energy diagram showing the absorption and emission of a photon by an atom or a molecule. When a photon of energy hν strikes the atom or molecule, absorption may occur if the difference in energy, … Continue reading