Tag Archives: capillary electrophoresis

Reversed Migration in Capillary Electrophoresis

We can reverse the direction of electroosmotic flow in capillary electrophoresis by adding an alkylammonium salt to the buffer solution. As shown in (b), the positively charged end of the alkyl ammonium ions bind to the negatively charged silanate ions … Continue reading

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Stacking of Cations and Anions in Capillary Electrophoresis

When a analyte’s concentration is too small to detect reliably, it may be possible to introduce it in a manner that increases its concentration in the capillary tube. This method of injection is called stacking. Stacking is accomplished by placing … Continue reading

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UV/Vis Detectors for Capillary Electrophoresis

Most of the detectors used in HPLC also find use in capillary electrophoresis. Among the more common detectors are those based on the absorption of UV/Vis radiation, fluorescence, conductivity, amperometry, and mass spectrometry. Whenever possible, detection is done “on-column” before … Continue reading

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Instrumentation for Capillary Electrophoresis

The basic instrumentation for capillary electrophoresis is shown here and includes a power supply for applying the electric field, anode and cathode compartments containing reservoirs of the buffer solution, a sample vial containing the sample, the capillary tube, and a … Continue reading

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Total Mobility of Solutes in Capillary Electrophoresis

In capillary electrophoresis we inject the sample into a buffered solution retained within a capillary tube. When an electric field is applied across the capillary tube, the sample’s components migrate as the result of two types of action: electrophoretic mobility, … Continue reading

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