Tag Archives: resolution

Optimizing Separation Using a Window Diagram

The illustration below shows how the mobile phase pH in liquid chromatography affects our ability to separate a mixture of four substituted benzoic acids.┬áThe retention times in (a) suggest there are many pH values where some separation is possible. To … Continue reading

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The General Elution Problem in Chromatography

Adjusting the retention factor to improve the resolution between one pair of solutes may lead to unacceptably long retention times for other solutes. For example, suppose we need to analyze a four-component mixture with baseline resolution and with a run-time … Continue reading

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Chromatographic Resolution

The goal of chromatography is to separate a mixture into a series of chromatographic peaks, each representing a single component of the mixture. The resolution between two chromatographic peaks, RAB, is a quantitative measure of their separation, and is defined … Continue reading

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