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Tag Archives: resolution
Optimizing Separation Using a Window Diagram
The illustration below shows how the mobile phase pH in liquid chromatography affects our ability to separate a mixture of four substituted benzoic acids. The retention times in (a) suggest there are many pH values where some separation is possible. To … Continue reading
Posted in Illustration
Tagged alpha, chromatography, HPLC, resolution, separation, window diagram
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The General Elution Problem in Chromatography
Adjusting the retention factor to improve the resolution between one pair of solutes may lead to unacceptably long retention times for other solutes. For example, suppose we need to analyze a four-component mixture with baseline resolution and with a run-time … Continue reading
Posted in Illustration
Tagged chromatography, general elution problem, resolution, separation
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Chromatographic Resolution
The goal of chromatography is to separate a mixture into a series of chromatographic peaks, each representing a single component of the mixture. The resolution between two chromatographic peaks, RAB, is a quantitative measure of their separation, and is defined … Continue reading