Tag Archives: UV/Vis

UV/Vis Detectors for Capillary Electrophoresis

Most of the detectors used in HPLC also find use in capillary electrophoresis. Among the more common detectors are those based on the absorption of UV/Vis radiation, fluorescence, conductivity, amperometry, and mass spectrometry. Whenever possible, detection is done “on-column” before … Continue reading

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Absorbance and Fluorescence Detection for HPLC Separations

One popular HPLC detector takes advantage of an analyte’s UV/Vis absorption spectrum. These detectors range from simple designs, in which the analytical wavelength is selected using appropriate filters, to a modified spectrophotometer in which the sample compartment includes a flow … Continue reading

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Sample Cells for UV/Vis and IR Spectroscopy

The photo below shows several examples of sample cells for UV/Vis spectroscopy. From left to right (with path lengths in parentheses): rectangular plastic cuvette (10.0 mm), rectangular quartz cuvette (5.000 mm), rectangular quartz cuvette (1.000 mm), cylindrical quartz cuvette (10.00 … Continue reading

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