The kinetics of the dephosphorylation of sodium p-nitrophenol using the enzyme bovine alkaline phosphatase is studied by measuring the reaction’s velocity spectrophotometrically as a function of the substrate’s concentration. The kinetic parameters Km and Vm are determined using Lineweaver-Burke and Eadie-Hofstee plots.
The full citation is here: Barton, J. J. Chem. Educ. 2011, 88, 1336–1339 and a link to the article is provided below (subscription to the journal required).
